Journal: British Journal of Cancer

Article Title: Inhibition of the AURKA/YAP1 axis is a promising therapeutic option for overcoming cetuximab resistance in colorectal cancer stem cells

doi: 10.1038/s41416-024-02649-z

Figure Lengend Snippet: a Plot representing the distribution of YAP1 expression in CRC cell lines sensitive to cetuximab compared to the resistant ones ns =non-significant * p < 0.05, two-tailed t-Student’s test. b Plot depicting the distribution of YAP1 activity score in cetuximab-sensitive CRC cell lines compared to the resistant ones. c Correlation analysis between AURKA expression and YAP1 activity score. d Correlation analysis between AURKA and YAP1 expression levels. e Western blot illustrating the basal levels of total amount and phosphorylated YAP1 (Ser397) in CRC cell lines HCA46, SW48 and C10. Tubulin was used as a loading control. Results are plotted as the average ± SD of all the biological replicates and were normalised to the HCA46 cell line ( n = 3). ns =non-significant, ** p < 0.01, *** p < 0.001, one-way ANOVA. f Gene expression levels of CTGF and CYR61 in CRC cell lines HCA46, SW48 and C10 ( n = 3). ns =non-significant, ** p < 0.01, *** p < 0.001, one-way ANOVA.

Article Snippet: Human CRC cell line HCA46 was obtained from European Collection of Authenticated Cell Cultures (ECACC).

Techniques: Expressing, Two Tailed Test, Activity Assay, Western Blot, Control, Gene Expression

Journal: British Journal of Cancer

Article Title: Inhibition of the AURKA/YAP1 axis is a promising therapeutic option for overcoming cetuximab resistance in colorectal cancer stem cells

doi: 10.1038/s41416-024-02649-z

Figure Lengend Snippet: a Western blot illustrating c-MET levels in the HCA46, SW48 and C10 cell lines. Tubulin was used as a loading control. Results are plotted as the average ± SD of all the biological replicates and were normalized to the HCA46 cell line ( n = 3). b Colony formation assay of CRC cell lines HCA46, SW48 and C10.Results are plotted as the average ± SD of all the biological replicates. ** p < 0.01 ( n = 3), one-way ANOVA. c ALDH1 relative activity in CRC cell lines HCA46, SW48 and C10. Results were normalized to the cetuximab-sensitive HCA46 cell line. Results are plotted as the average ± SD of all the biological replicates. ** p < 0.01, *** p < 0.001, one-way ANOVA. d Western blot illustrating c-MET expression in SW48 and C10 cell line transduced with YAP1 and YAP1 S397A plasmids, along with the empty vector. Results were normalized to the YAP1-trasduced cell lines. e Colony formation assay of SW48 and C10 cell line transduced with YAP1 and YAP1 S397A plasmids, along with the empty vector. Results are plotted as the average ± SD of all the biological replicates, ** p < 0.01 ( n = 3), one-way ANOVA. f ALDH1 relative activity in SW48 and C10 cell line transduced with YAP1 and YAP1 S397A plasmids, along with the empty vector. Results were normalized to the empty vector condition in each cell line. Results are plotted as the average ± SD of all the biological replicates, * p < 0.05, ** p < 0.01 ( n = 3), one-way ANOVA. g SOX2 gene expression in SW48 and C10 cell line transduced with YAP1 and YAP1 S397A plasmids, along with the empty vector. Results were normalized to the empty vector condition in each cell line. Results are plotted as the average ± SD of all the biological replicates * p < 0.05, ** p < 0.01 ( n = 3), one-way ANOVA.

Article Snippet: Human CRC cell line HCA46 was obtained from European Collection of Authenticated Cell Cultures (ECACC).

Techniques: Western Blot, Control, Colony Assay, Activity Assay, Expressing, Transduction, Plasmid Preparation, Gene Expression

Journal: Gut

Article Title: Circulating microRNA-203 predicts prognosis and metastasis in human colorectal cancer

doi: 10.1136/gutjnl-2014-308737

Figure Lengend Snippet: (A) Expression status of miR-203 in 58 pairs of primary CRC (pCRC) and corresponding matching liver metastasis (LM). The gray horizontal bars represent mean expression levels; ***P<0.001, t test. (B) In situ hybridization analysis of miR-203 expression in pCRC and corresponding LMs. (Positive control, U6 snRNA; negative control, scrambled miRNA control) (C) Expression of miR-203 in CRC cell lines (CaCO2, HCT116, HT29, LoVo, SW480 and SW620). (D) The amount of miR-203 excreted in the HT-29 culture medium increased depending on cell number and duration of culture (E), whereas these levels decreased following treatment with miR-203 inhibitor in HT-29 cells (F). Serum miR-203 levels in healthy control subjects (N; n = 24) and different Tumor Node Metastasis (TNM) stages (I, II, III, and IV) of CRC (n = 186) in Cohort 1. ***P<0.001, *P<0.05 (G). Tissue miR-203 levels in different Tumor Node Metastasis (TNM) stages (I,II,III, and IV) of CRCs (n=154) and adjacent normal mucosa (N; n = 20) in Cohort 1. NS: not significant, ***P<0.001.

Article Snippet: With regard to systemic metastasis models, CRC cells (1.0 × 10 6 /200 μL of PBS) were injected into the tail vein of six week old male athymic nude mice (Balb/c nu; Harlan Laboratories, Inc.).

Techniques: Expressing, In Situ Hybridization, Positive Control, Negative Control, Control

Journal: Gut

Article Title: Circulating microRNA-203 predicts prognosis and metastasis in human colorectal cancer

doi: 10.1136/gutjnl-2014-308737

Figure Lengend Snippet: Clinical Significance of Tissue and Serum miR-203 Expression in a Matched Tissues and Serum in Cohort 1 CRC patients *

Article Snippet: With regard to systemic metastasis models, CRC cells (1.0 × 10 6 /200 μL of PBS) were injected into the tail vein of six week old male athymic nude mice (Balb/c nu; Harlan Laboratories, Inc.).

Techniques: Expressing

Journal: Gut

Article Title: Circulating microRNA-203 predicts prognosis and metastasis in human colorectal cancer

doi: 10.1136/gutjnl-2014-308737

Figure Lengend Snippet: Overall survival analyses based on miR-203 expression of 186 serum specimens and 154 matched tissue specimens from CRC patients. (A) Overall survival of CRC patients (all stage) based on serum miR-203 expression. (B) Overall survival of all CRC patients (all stage) based on tissue miR-203 expression. (C) Overall survival of curative CRC patients (stage I-III) based on serum miR-203 expression. (D) Overall survival of non-curative CRC patients (stage IV) based on serum miR-203 expression. Serum miR-203 expression levels based on the Japanese Society for Cancer of the Colon and Rectum (JSCCR) classification system; (E) lymph node N-classification (N0, absent lymph node metastasis; N1, metastasis in 1–3 regional lymph nodes; N2, metastasis in 4 or more regional lymph nodes; N3, lymph node metastasis to the aorta), (F) liver metastasis H-classification (H0, no liver metastasis; H1, liver metastasis with less than 5 nodules smaller than 5 cm; H2, metastasis that does not involve H1 and H3; H3, liver metastasis with more than 5 metastasis larger than 5 cm), and (G) Peritoneal metastasis P-classification (P0, no peritoneal metastasis; P1, metastasis localized to the adjacent peritoneum; P2, limited metastasis to the distant peritoneum; P3, diffuse metastasis to the distant peritoneum).

Article Snippet: With regard to systemic metastasis models, CRC cells (1.0 × 10 6 /200 μL of PBS) were injected into the tail vein of six week old male athymic nude mice (Balb/c nu; Harlan Laboratories, Inc.).

Techniques: Expressing

Journal: Gut

Article Title: Circulating microRNA-203 predicts prognosis and metastasis in human colorectal cancer

doi: 10.1136/gutjnl-2014-308737

Figure Lengend Snippet: Association Between miR-203 Expression and Prognosis in Cohort 1 CRC patients

Article Snippet: With regard to systemic metastasis models, CRC cells (1.0 × 10 6 /200 μL of PBS) were injected into the tail vein of six week old male athymic nude mice (Balb/c nu; Harlan Laboratories, Inc.).

Techniques: Expressing

Journal: Gut

Article Title: Circulating microRNA-203 predicts prognosis and metastasis in human colorectal cancer

doi: 10.1136/gutjnl-2014-308737

Figure Lengend Snippet: Association between miR-203 Expression and metastasis prediction in Cohort 1 CRC patients

Article Snippet: With regard to systemic metastasis models, CRC cells (1.0 × 10 6 /200 μL of PBS) were injected into the tail vein of six week old male athymic nude mice (Balb/c nu; Harlan Laboratories, Inc.).

Techniques: Expressing

Journal: Gut

Article Title: Circulating microRNA-203 predicts prognosis and metastasis in human colorectal cancer

doi: 10.1136/gutjnl-2014-308737

Figure Lengend Snippet: Athymic nude mice for liver metastatic models were sacrificed 10 weeks after injection of HT-29 CRC cells (2.0 × 106/50 μL of PBS) in the spleen. (A) Representative images of non-treated control mouse. (B) Representative images of animals with established liver metastasis. Yellow arrows indicate metastatic foci. The upper-right panel illustrates representative results of H&E staining in mouse liver. The lower-left panel displays representative results for ISH staining for miR-203 in animal liver. (C) miR-203 expression status in spleen (S), adjacent hepatocytes (H), and liver metastasis (LM) tissues of liver metastasis established mouse group (n=10; *** P<0.001). (D) Serum miR-203 expression status between non-treated control animals (non-LM; n=7) and animals with established liver metastasis (LM; n=10; * P<0.05). Athymic nude mice for systemic metastatic models were sacrificed 8 weeks after injection of HT-29 CRC cells (1.0 × 106/200 μL of PBS) in the tail vein. Representative images of non-treated controls (E) and established systemic metastatic mouse (F). Red arrows indicate metastatic foci. (G) Serum miR-203 expression status between non-treated control animals (non-SM; n=6) and animals with established systemic metastasis (SM; n=6; ** P<0.01). (H) Significant correlation between serum miR-203 expression and tumor volume in mice (Rho = 0.829, P=0.0009).

Article Snippet: With regard to systemic metastasis models, CRC cells (1.0 × 10 6 /200 μL of PBS) were injected into the tail vein of six week old male athymic nude mice (Balb/c nu; Harlan Laboratories, Inc.).

Techniques: Injection, Control, Staining, Expressing